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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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Thermo Fisher quantitative peroxide assay kit thermo scientific #23280
(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and <t>H2O2</t> content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).
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(A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and H2O2 content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).

Journal: PeerJ

Article Title: Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses

doi: 10.7717/peerj.17462

Figure Lengend Snippet: (A) Plant albino phenotypes of TRV::CLA. (B, C) Phenotypes of TRV::00 and TRV:: GhCKX34A before cold stress. (D) GhCKX34A expression levels in leaves of TRV::00 and TRV:: GhCKX34A plants. (E, F) Phenotypes of TRV::00 and TRV:: GhCKX34A after cold treatment for 24 h. (G, H) The MDA and H2O2 content of TRV::00 and TRV:: GhCKX34A after cold stress. * p < 0.05, ** p < 0.01, and *** p < 0.001 (Student’s one-tailed t test).

Article Snippet: Malondialdehyde (MDA) and hydrogen peroxide (H2O2) content were quantified using the MDA (EEA015) and H2O2 (23280) assay kits (Thermo Fisher Scientific).

Techniques: Expressing, One-tailed Test